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ATG9A Monoklonaler Antikörper

ATG9A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D12E11

Kat-Nr. : 67096-1-PBS

Synonyme

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

Filter:
  • Western Blot (WB) analysis of various lysates using ATG9A Monoclonal antibody (67096-1-Ig)

    WB analysis using 67096-1-Ig (same clone as 67096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67096-1-Ig (ATG9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using ATG9A Monoclonal antibody (67096-1-Ig)

    IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using ATG9A Monoclonal antibody (67096-1-Ig)

    IHC staining of human thyroid cancer using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATG9A Monoclonal antibody (67096-1-Ig)

    IF Staining of HepG2 using 67096-1-Ig (same clone as 67096-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67096-1-Ig (ATG9A antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67096-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67096-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATG9A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ATG9A fusion protein Ag24278
Vollständiger Name ATG9 autophagy related 9 homolog A (S. cerevisiae)
Berechnetes Molekulargewicht 837 aa, 94 kDa
Beobachtetes Molekulargewicht 94 kDa
GenBank-ZugangsnummerBC065534
Gene symbol ATG9A
Gene ID (NCBI) 79065
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)