ATGL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATGL Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

45

Rezensionen (2)

Kat-Nr. : 55190-1-AP

Synonyme

1110001C14Rik, Adipose Triglyceride Lipase, ATGL, DESNUTRIN, FP17548, IPLA2 zeta, PEDF R, PNPLA2, Transport secretion protein 2, TTS 2.2, TTS2, TTS2.2

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Galerie der Validierungsdaten

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  WB analysis of 3T3-L1 using 55190-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55190-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55190-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 55190-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55190-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 55190-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55190-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 55190-1-AP

  IP Result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, A431-Zellen, HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Maushodengewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 41 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

55190-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ATGL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: patatin-like phospholipase domain containing 2
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_020376
• Gene symbol: PNPLA2
• Gene ID (NCBI): 57104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

Publikationen

Species	Application	Title
mouse	WB	J Pineal Res; Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin.; Authors - Zhenhui Li
human		Hepatology; PMID: 29729186; Authors - Huixia Zhang
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	Food Chem; Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity.; Authors - Shanshan Hu
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr-/- mice; Authors - Kenan Peng
rat	WB,IF	Int J Mol Med; PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF‑R.; Authors - Qixiang Zhao ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Li (Verified Customer) (07-12-2019)

We have tried a couple different ATGL antibodies from other vendors, but no one is working as good as we want. So we tried this one, it works great.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse muscle

FH

Shantel (Verified Customer) (05-02-2019)

Works well in Western Blot. I used mouse liver tissue, 40ug/ul protein per lane at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver

ATGL Antibody Western Blot, validation (1:1000 dilution) in mouse liver (Cat no:55190-1-AP)