ATGL Polyklonaler Antikörper

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• KD/KO Validated

ATGL Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55190-1-PBS

Synonyme

Adipose triglyceride lipase, PNPLA2, Calcium-independent phospholipase A2-zeta, Desnutrin, EC:3.1.1.3

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  WB analysis of 3T3-L1 using 55190-1-AP (same clone as 55190-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 55190-1-AP (same clone as 55190-1-PBS)

  WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 55190-1-AP (same clone as 55190-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 55190-1-AP (same clone as 55190-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 55190-1-AP (same clone as 55190-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55190-1-AP (same clone as 55190-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 55190-1-AP (same clone as 55190-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 55190-1-AP (same clone as 55190-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 55190-1-AP (same clone as 55190-1-PBS)

  IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55190-1-PBS bindet in WB, IP, Indirect ELISA ATGL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: patatin-like phospholipase domain containing 2
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: NM_020376
• Gene symbol: ATGL
• Gene ID (NCBI): 57104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.