ATIC Rekombinanter Antikörper

ATIC Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242324C8

Kat-Nr. : 84798-5-PBS

Synonyme

242324C8, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR, AICAR formyltransferase, AICAR transformylase/inosine monophosphate cyclohydrolase

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  WB analysis using 84798-5-RR (same clone as 84798-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84798-5-RR (ATIC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84798-5-RR (same clone as 84798-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84798-5-RR (ATIC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84798-5-RR (same clone as 84798-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATIC antibody (84798-5-RR, Clone: 242324C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84798-5-RR (same clone as 84798-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATIC Recombinant antibody (84798-5-RR, Clone:242324C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84798-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84798-5-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA ATIC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATIC fusion protein Ag1052
• Vollständiger Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC008879
• Gene symbol: ATIC
• Gene ID (NCBI): 471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC, also known as AICAR) is an enzyme that can catalyze the last two reactions in the de novo purine biosynthetic pathway. It has been previously reported to be upregulated and to participate in myeloma and hepatocellular carcinoma progression (PMID: 35251351).