ATL3 Polyklonaler Antikörper

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ATL3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16921-1-PBS

Synonyme

EC:3.6.5.-, Atlastin-3, Atlastin 3

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  WB analysis using 16921-1-AP (same clone as 16921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

  WB result of ATL3 antibody (16921-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected HeLa cells. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis using 16921-1-AP (same clone as 16921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16921-1-AP (same clone as 16921-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16921-1-AP (same clone as 16921-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16921-1-AP (same clone as 16921-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 16921-1-AP (same clone as 16921-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

  IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

  IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:8000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 16921-1-AP (same clone as 16921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (16921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16921-1-AP (same clone as 16921-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16921-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ATL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATL3 fusion protein Ag10279
• Vollständiger Name: atlastin GTPase 3
• Berechnetes Molekulargewicht: 541 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC077727
• Gene symbol: ATL3
• Gene ID (NCBI): 25923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.