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ATL3 Monoklonaler Antikörper

ATL3 Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E7C4

Kat-Nr. : 68516-1-PBS

Synonyme

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

Filter:
  • Western Blot (WB) analysis of various lysates using ATL3 Monoclonal antibody (68516-1-Ig)

    WB analysis using 68516-1-Ig (same clone as 68516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ATL3 Monoclonal antibody (68516-1-Ig)

    WB analysis of Jurkat using 68516-1-Ig (same clone as 68516-1-PBS)

    WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATL3 Monoclonal antibody (68516-1-Ig)

    IF Staining of HeLa using 68516-1-Ig (same clone as 68516-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (68516-1-Ig, Clone: 2E7C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50703-1

    Cytometric bead array standard curve of MP50703-1

    Cytometric bead array standard curve of MP50703-1, ATL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68516-2-PBS. Detection antibody: 68516-1-PBS. Standard:Ag10248. Range: 0.781-100 ng/mL.

Geprüfte Anwendungen

Produktinformation

68516-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA ATL3 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

Getestete Reaktivität human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ATL3 fusion protein Ag10248
Vollständiger Name atlastin GTPase 3
Berechnetes Molekulargewicht 541 aa, 61 kDa
Beobachtetes Molekulargewicht 61 kDa
GenBank-ZugangsnummerBC077727
Gene symbol ATL3
Gene ID (NCBI) 25923
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.