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ATL3 Rekombinanter Antikörper

ATL3 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241937G3

Kat-Nr. : 84578-3-PBS

Synonyme

241937G3, Atlastin 3, Atlastin-3, EC:3.6.5.-

Filter:
  • Western Blot (WB) analysis of various lysates using ATL3 Recombinant antibody (84578-3-RR)

    WB analysis using 84578-3-RR (same clone as 84578-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84578-3-RR (ATL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84578-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATL3 Recombinant antibody (84578-3-RR)

    IF Staining of HeLa using 84578-3-RR (same clone as 84578-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (84578-3-RR, Clone: 241937G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84578-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84578-3-RR

    Affinity and Kinetic Characterization of 84578-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84578-3-RR against Human ATL3 were performed. The affinity constant is 1.14 nM.

Geprüfte Anwendungen

Produktinformation

84578-3-PBS bindet in WB, IF/ICC, Indirect ELISA ATL3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ATL3 fusion protein Ag10248
Vollständiger Name atlastin GTPase 3
Berechnetes Molekulargewicht 541 aa, 61 kDa
Beobachtetes Molekulargewicht 58-61 kDa
GenBank-ZugangsnummerBC077727
Gene symbol ATL3
Gene ID (NCBI) 25923
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.