ATM Rekombinanter Antikörper

ATM Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240560H8

Kat-Nr. : 83608-4-PBS

Synonyme

240560H8, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

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  Sandwich ELISA standard curve of MP00592-4

  Sandwich ELISA standard curve of MP00592-4, Human ATM Recombinant Matched Antibody Pair - PBS only. 83608-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25920. 83608-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP00592-4

  The mean ATM concentration was determined to be 3,383.80 pg/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 3,536.69 pg/mL in MDA-MB-468 cell extract based on a 3.20 mg/mL extract load.

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  IF Staining of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-4-RR, Clone: 240560H8 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83608-4-RR (same clone as 83608-4-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83608-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM.

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Geprüfte Anwendungen

Produktinformation

83608-4-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ATM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATM fusion protein Ag25920
• Vollständiger Name: ataxia telangiectasia mutated
• GenBank-Zugangsnummer: BC137169
• Gene symbol: ATM
• Gene ID (NCBI): 472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.