ATOX1 Polyklonaler Antikörper

ATOX1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22641-1-PBS

Synonyme

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  WB analysis using 22641-1-AP (same clone as 22641-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22641-1-AP (same clone as 22641-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 22641-1-AP (same clone as 22641-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 22641-1-AP (same clone as 22641-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 22641-1-AP (same clone as 22641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22641-1-AP (ATOX1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 22641-1-AP (same clone as 22641-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATOX1 antibody (22641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22641-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATOX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATOX1 fusion protein Ag18460
• Vollständiger Name: ATX1 antioxidant protein 1 homolog (yeast)
• Berechnetes Molekulargewicht: 68 aa, 8 kDa
• Beobachtetes Molekulargewicht: 7 kDa
• GenBank-Zugangsnummer: BC112248
• Gene symbol: ATOX1
• Gene ID (NCBI): 475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).