ATOX1 Rekombinanter Antikörper

ATOX1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240679E9

Kat-Nr. : 83785-6-PBS

Synonyme

ATX1, ATOX 1, Antioxidant 1, 240679E9

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  WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83785-6-RR (ATOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83785-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83785-6-RR against Human ATOX1 were performed. The affinity constant is 183 nM.

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Geprüfte Anwendungen

Produktinformation

83785-6-PBS bindet in WB, FC (Intra), ELISA ATOX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATOX1 fusion protein Ag18460
• Vollständiger Name: ATX1 antioxidant protein 1 homolog (yeast)
• Berechnetes Molekulargewicht: 68 aa, 8 kDa
• Beobachtetes Molekulargewicht: 7 kDa
• GenBank-Zugangsnummer: BC112248
• Gene symbol: ATOX1
• Gene ID (NCBI): 475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).