ATP11B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP11B Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 13672-1-AP

Synonyme

Phospholipid-transporting ATPase IF, P4-ATPase flippase complex alpha subunit ATP11B, EC:7.6.2.1, ATPIR, ATPIF

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  IHC staining of human ovary tumor using 13672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13672-1-AP (ATP11B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 13672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13672-1-AP (ATP11B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 13672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13672-1-AP (ATP11B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 13672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13672-1-AP (ATP11B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 13672-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP11B antibody (13672-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13672-1-AP bindet in WB, IHC, IF/ICC, ELISA ATP11B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP11B fusion protein Ag4654
• Vollständiger Name: ATPase, class VI, type 11B
• Berechnetes Molekulargewicht: 1177aa, 134 kDa
• GenBank-Zugangsnummer: BC033880
• Gene symbol: ATP11B
• Gene ID (NCBI): 23200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IF	Acta Neuropathol; Loss of the heterogeneous expression of flippase ATP11B leads to cerebral small vessel disease in a normotensive rat model.; Authors - Sophie Quick
human,mouse	WB,IHC	J Immunother Cancer; Oncolytic peptide LTX-315 induces anti-pancreatic cancer immunity by targeting the ATP11B-PD-L1 axis.; Authors - Tianyu Tang ; KO Validated
rat	WB	Life Sci; miRNA-150-5p associate with antihypertensive effect of epigallocatechin-3-gallate revealed by aorta miRNome analysis of spontaneously hypertensive rat.; Authors - Bing-Jun Qian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ruby (Verified Customer) (06-01-2025)

I never got any selective staining.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 1000 (WB), 1 in 400 (IF)
• Cell Tissue Type: Platelets