ATP1A1/2 Monoklonaler Antikörper

ATP1A1/2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D8G1

Kat-Nr. : 68505-1-PBS

Synonyme

ATP1A2, 3D8G1, alpha 2 ATPase, ATP1A 2, EC:7.2.2.13

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  WB analysis using 68505-1-Ig (same clone as 68505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of SK-BR-3 using 68505-1-Ig (same clone as 68505-1-PBS)

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 68505-1-Ig (same clone as 68505-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 68505-1-Ig (same clone as 68505-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 68505-1-Ig (same clone as 68505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:450 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68505-1-Ig (same clone as 68505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68505-1-PBS bindet in WB, IF/ICC, Indirect ELISA ATP1A1/2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP1A1/2 fusion protein Ag10489
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Berechnetes Molekulargewicht: 1020 aa, 112 kDa
• Beobachtetes Molekulargewicht: 97-100 kDa
• GenBank-Zugangsnummer: BC052271
• Gene symbol: ATP1A2
• Gene ID (NCBI): 477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.