ATP1A1 Monoklonaler Antikörper

ATP1A1 Monoklonal Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9B7

Kat-Nr. : 68735-2-PBS

Synonyme

1E9B7, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

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  WB analysis using 68735-2-Ig (same clone as 68735-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68735-2-Ig (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50088-1

  Sandwich ELISA standard curve of MP50088-1, ATP1A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68735-1-PBS. Detection antibody: HRP-conjugated 68735-2-PBS. Standard:Ag5990. Range: 0.195-50 ng/mL.

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  IHC staining of mouse kidney using 68735-2-Ig (same clone as 68735-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68735-2-Ig (same clone as 68735-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68735-2-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA ATP1A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP1A1 fusion protein Ag5990
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC050359
• Gene symbol: ATP1A1
• Gene ID (NCBI): 476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.