ATP1A2 Rekombinanter Antikörper

ATP1A2 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242414G12

Kat-Nr. : 84999-4-PBS

Synonyme

242414G12, alpha 2 ATPase, ATP1A 2, EC:7.2.2.13, Na(+)/K(+) ATPase alpha-2 subunit

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  WB analysis using 84999-4-RR (same clone as 84999-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84999-4-RR (ATP1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84999-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84999-4-RR (same clone as 84999-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84999-4-RR (ATP1A2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84999-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84999-4-PBS bindet in WB, IHC, Indirect ELISA ATP1A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP1A2 fusion protein Ag10489
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Berechnetes Molekulargewicht: 1020 aa, 112 kDa
• Beobachtetes Molekulargewicht: 97-100 kDa
• GenBank-Zugangsnummer: BC052271
• Gene symbol: ATP1A2
• Gene ID (NCBI): 477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4. The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. For optimal WB detection with ATP1A2, we do not recommend boiling the sample after lysis.