ATP1B1 Rekombinanter Antikörper

ATP1B1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242109D12

Kat-Nr. : 84707-5-RR

Synonyme

242109D12, ATP1B 1, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

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  WB analysis using 84707-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84707-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84707-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 84707-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of mouse kidney using 84707-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:1000 (under 40x lens).

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  Affinity and Kinetic Characterization of 84707-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84707-5-RR bindet in WB, IHC, ELISA ATP1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP1B1 fusion protein Ag7279
• Vollständiger Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 45-52 kDa
• GenBank-Zugangsnummer: BC000006
• Gene symbol: ATP1B1
• Gene ID (NCBI): 481
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)