ATP1B3 Monoklonaler Antikörper

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ATP1B3 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H9C1

Kat-Nr. : 67554-1-PBS

Synonyme

1H9C1, ATPB 3, ATPB-3, CD298, Sodium/potassium-dependent ATPase subunit beta-3

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  WB analysis of Jurkat using 67554-1-Ig (same clone as 67554-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67554-1-Ig (same clone as 67554-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67554-1-Ig (same clone as 67554-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67554-1-Ig (same clone as 67554-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67554-1-Ig (same clone as 67554-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1B3 antibody (67554-1-Ig, Clone: 1H9C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67554-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATP1B3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP1B3 fusion protein Ag30127
• Vollständiger Name: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32-43 kDa
• GenBank-Zugangsnummer: BC011835
• Gene symbol: ATP1B3
• Gene ID (NCBI): 483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).