ATP2A1 Polyklonaler Antikörper

ATP2A1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22361-1-PBS

Synonyme

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  WB analysis using 22361-1-AP (same clone as 22361-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22361-1-AP (ATP2A1 antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 22361-1-AP (same clone as 22361-1-PBS)

  IP result of anti-ATP2A1 (IP:22361-1-AP, 5ug; Detection:22361-1-AP 1:700) with mouse skeletal muscle tissue lysate 2160ug. This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 22361-1-AP (same clone as 22361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 22361-1-AP (same clone as 22361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 22361-1-AP (same clone as 22361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22361-1-AP (ATP2A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 22361-1-AP (same clone as 22361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22361-1-AP (ATP2A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22361-1-AP (same clone as 22361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22361-1-AP (same clone as 22361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22361-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22361-1-PBS bindet in WB, IHC, IP, Indirect ELISA ATP2A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP2A1 fusion protein Ag17944
• Vollständiger Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Berechnetes Molekulargewicht: 1001 aa, 110 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC037354
• Gene symbol: ATP2A1
• Gene ID (NCBI): 487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).