ATP2B1 Rekombinanter Antikörper

ATP2B1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240848D10

Kat-Nr. : 83827-3-PBS

Synonyme

240848D10, PMCA1, PMCA1kb

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  WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83827-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83827-3-PBS against Human ATP2B1 was performed. The affinity constant is 4.46 nM.

• 

  Affinity and Kinetic Characterization of 83827-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

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Geprüfte Anwendungen

Produktinformation

83827-3-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA ATP2B1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP2B1 fusion protein Ag32423
• Vollständiger Name: ATPase, Ca++ transporting, plasma membrane 1
• Berechnetes Molekulargewicht: 135 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_001001323.1
• Gene symbol: ATP2B1
• Gene ID (NCBI): 490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).