ATP5A1 Monoklonaler Antikörper

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• KD/KO Validated

ATP5A1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(33)

CloneNo.

1B10H3

Rezensionen (4)

Kat-Nr. : 66037-1-Ig

Synonyme

1B10H3, ATP synthase F1 subunit alpha, ATP5A, ATP5A 1

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  WB analysis using 66037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66037-1-Ig

  WB result of ATP5A1 antibody (66037-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5A1 transfected HepG2 cells.

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  WB analysis of HepG2 using 66037-1-Ig

  Western blot analysis of ATP5A1 in various tissues and cell lines using Proteintech antibody 66037-1-Ig at the dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66037-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66037-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 66037-1-Ig

  IP result of anti-ATP5A1 (IP:66037-1-Ig, 5ug; Detection:66037-1-Ig 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66037-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin. .

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  IF Staining of HeLa using 66037-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (66037-1-Ig, Clone: 1B10H3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66037-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (66037-1-Ig, Clone: 1B10H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  IF Staining of HeLa using 66037-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Produktinformation

66037-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA ATP5A1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP5A1 fusion protein Ag8119
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC064562
• Gene symbol: ATP5A1
• Gene ID (NCBI): 498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Publikationen

Species	Application	Title
human	IF	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu
mouse	WB	Circ Res; Endothelial FUNDC1 Deficiency Drives Pulmonary Hypertension; Authors - Yandong Pei
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
hamster	IF	Nat Commun; The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters; Authors - Xiaolong Lv
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anis (Verified Customer) (06-26-2025)

Jess - SimpleWestern, 1:40 in antibody diluent 2 Used as a marker for mitochondria isolation purity

• Applications: Western Blot
• Primary Antibody Dilution: 1:40
• Cell Tissue Type: A549/mitochondria isolate

ATP5A1 Antibody Western Blot validation (1:40 dilution) in A549/mitochondria isolate (Cat no:66037-1-Ig)

FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/antibody dilution solution: 0.05% saponin, 5% horse serum in PBS. Primary antibody diluted 1:100 in saponin solution for 1hr at room temperature, followed by anti-mouse conjugated to AlexaFluor 568 (1:200 in saponin solution) for 1 hr at room temperature. I got very low signal and a lot of background specks, so this antibody does not work well with the saponin permeabilization.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231 cells

ATP5A1 Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:66037-1-Ig)

FH

Marina (Verified Customer) (09-12-2022)

Mitochondrial fractions were obtained from whole cell lysates of 2 immortalized human breast cancer cell lines using a commercial kit, leading to pure mitochondrial fractions (ATP5A1 positive) and fractions comprising the cytosols minus the mitochondria (ATP5A1 negative). Primary antibody incubation was performed overnight at 4ºC. Ponceau S is used as total protein loading control.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human breast cancer cells

ATP5A1 Antibody Western Blot validation (1:5000 dilution) in Human breast cancer cells (Cat no:66037-1-Ig)

FH

Cassandr (Verified Customer) (02-10-2022)

Consistently works antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HULEC

ATP5A1 Antibody Immunofluorescence validation (1:250 dilution) in HULEC (Cat no:66037-1-Ig)