ATP5A1 Rekombinanter Antikörper

ATP5A1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4O12

Kat-Nr. : 82288-1-PBS

Synonyme

ATP5A 1, ATP5A, ATP synthase F1 subunit alpha, 4O12

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  WB analysis using 82288-1-RR (same clone as 82288-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82288-1-RR (ATP5A1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82288-1-RR (same clone as 82288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82288-1-RR (same clone as 82288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82288-1-RR (same clone as 82288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82288-1-RR (same clone as 82288-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82288-1-RR (ATP5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82288-1-RR (same clone as 82288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82288-1-RR (same clone as 82288-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82288-1-RR (same clone as 82288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82288-1-RR (same clone as 82288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82288-1-RR (same clone as 82288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5A1 antibody (82288-1-RR, Clone: 4O12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82288-1-RR (same clone as 82288-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5A1 (82288-1-RR, Clone:4O12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82288-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82288-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82288-1-RR against Human ATP5A1 were performed. The affinity constant is 2.37 nM.

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Geprüfte Anwendungen

Produktinformation

82288-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA ATP5A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP5A1 fusion protein Ag6385
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC064562
• Gene symbol: ATP5A1
• Gene ID (NCBI): 498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.