ATP5C1 Polyklonaler Antikörper

ATP5C1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10910-1-AP

Synonyme

ATP5C, ATP5C1, ATP5CL1, ATPG, F ATPase gamma subunit

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Galerie der Validierungsdaten

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  WB analysis using 10910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10910-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 10910-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red).

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  IF Staining of HepG2 using 10910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

10910-1-AP bindet in WB, IP, IHC, IF, ELISA ATP5C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5C1 fusion protein Ag1343
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000931
• Gene symbol: ATP5C1
• Gene ID (NCBI): 509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).

Publikationen

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IP	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	PLoS One; Warburg effect's manifestation in aggressive pheochromocytomas and paragangliomas: insights from a mouse cell model applied to human tumor tissue.; Authors - Stephanie M J Fliedner
human	WB	FEBS Lett; Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.; Authors - Makoto Fujikawa