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ATP5E Polyklonaler Antikörper

ATP5E Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15408-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ATP5E Polyclonal antibody (15408-1-AP)

    WB analysis using 15408-1-AP (same clone as 15408-1-PBS)

    Boiled and 37℃ incubated mouse heart tissue were subjected to SDS PAGE followed by western blot with 15408-1-AP (ATP5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15408-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5E Polyclonal antibody (15408-1-AP)

    IF Staining of HeLa using 15408-1-AP (same clone as 15408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15408-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP5E Polyclonal antibody (15408-1-AP)

    IF Staining of HeLa using 15408-1-AP (same clone as 15408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15408-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15408-1-PBS bindet in WB, IF/ICC, Indirect ELISA ATP5E und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ATP5E fusion protein Ag7672
Vollständiger Name ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Berechnetes Molekulargewicht 6 kDa
Beobachtetes Molekulargewicht6 kDa
GenBank-ZugangsnummerBC001690
Gene symbol ATP5E
Gene ID (NCBI) 514
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP synthase epsilon subunit (ATP5E) is an important subunit of ATP synthase, which is located in the stalk region of the F1 sector. F1-ATPase is the catalytic portion of mitochondrial ATP synthase, which produces ATP from ADP and inorganic phosphate (Pi). ATP5E is widely expressed in tissues. The calculated molecular weight of ATP5E is 6 kDa (PMID: 36625260).