ATP5F1 Monoklonaler Antikörper

ATP5F1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10G9

Kat-Nr. : 68304-1-PBS

Synonyme

ATP5F1, ATPase subunit b, PIG47

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  WB analysis using 68304-1-Ig (same clone as 68304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68304-1-Ig (ATP5F1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68304-1-Ig (same clone as 68304-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (68304-1-Ig, Clone: 1A10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68304-1-PBS bindet in WB, IF/ICC, Indirect ELISA ATP5F1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP5F1 fusion protein Ag8571
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC005366
• Gene symbol: ATP5F1
• Gene ID (NCBI): 515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.