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ATP5H Rekombinanter Antikörper

ATP5H Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250751G4

Kat-Nr. : 86174-2-PBS

Synonyme

ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ

Filter:
  • Western Blot (WB) analysis of various lysates using ATP5H Recombinant monoclonal antibody (86174-2-RR)

    WB analysis using 86174-2-RR (same clone as 86174-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86174-2-RR (ATP5H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATP5H Recombinant monoclonal antibody (86174-2-RR)

    IF Staining of HepG2 using 86174-2-RR (same clone as 86174-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5H antibody (86174-2-RR, Clone: 250751G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86174-2-RR

    Affinity and Kinetic Characterization of 86174-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86174-2-RR against Human ATP5H were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

86174-2-PBS bindet in WB, IF/ICC, Indirect ELISA ATP5H und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ATP5H fusion protein Ag11429
Vollständiger Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Berechnetes Molekulargewicht 137 aa, 16 kDa
Beobachtetes Molekulargewicht 19-22 kDa
GenBank-ZugangsnummerBC032245
Gene symbol ATP5H
Gene ID (NCBI) 10476
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.