ATP5H Rekombinanter Antikörper

ATP5H Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250751G4

Kat-Nr. : 86174-2-RR

Synonyme

ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ

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  WB analysis using 86174-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86174-2-RR (ATP5H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86174-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5H antibody (86174-2-RR, Clone: 250751G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86174-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86174-2-RR against Human ATP5H were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Ramos-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86174-2-RR bindet in WB, IF/ICC, ELISA ATP5H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP5H fusion protein Ag11429
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• Berechnetes Molekulargewicht: 137 aa, 16 kDa
• Beobachtetes Molekulargewicht: 19-22 kDa
• GenBank-Zugangsnummer: BC032245
• Gene symbol: ATP5H
• Gene ID (NCBI): 10476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.