ATP5J Polyklonaler Antikörper

ATP5J Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 14114-1-AP

Synonyme

ATP5PF, ATP synthase-coupling factor 6, mitochondrial, ATP synthase peripheral stalk subunit F6

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  WB analysis using 14114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14114-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14114-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14114-1-AP

  IP result of anti-ATP5J (IP:14114-1-AP, 4ug; Detection:14114-1-AP 1:300) with HEK-293 cells lysate 3680ug.

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  IHC staining of human osteosarcoma using 14114-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 14114-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5J antibody (14114-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U-251 using 14114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ATP5J antibody (14114-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Herzgewebe, Mauslebergewebe, SKOV-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Osteosarkomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

14114-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ATP5J und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5J fusion protein Ag5263
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 9 kDa
• GenBank-Zugangsnummer: BC066310
• Gene symbol: ATP5J
• Gene ID (NCBI): 522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5J, also known as coupling factor 6 (CF6), is a soluble integral component of mitochondrial ATP synthase. Mitochondrial ATP synthase is a multi-subunit membrane-bound enzyme that catalyzes the synthesis of ATP by utilizing a proton electrochemical gradient. It consists of three domains, namely the extrinsic and intrinsic membrane domains (F1 and F0, respectively) joined by a stalk. CF6 is one of the subunits in the stalk and an essential component for energy transduction. Recently CF6 has also been reported to play a crucial role in the development of INS resistance and hypertension. CF6 is first synthesized as an immature form in the cytosol, then transported to the mitochondria by an import signal peptide and becomes an active form with the signal peptide cleaved. Western blot analysis of CF6 demonstrates a single band around 9 kDa to 12 kDa in various tissues including heart, liver, brain and HUVEC (human umbilical vein endothelial cells).

Publikationen

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.; Authors - Linyi Song
mouse	WB	Arch Pharm Res; Overexpressing microRNA-203 alleviates myocardial infarction via interacting with long non-coding RNA MIAT and mitochondrial coupling factor 6.; Authors - Fan Wang
rabbit	WB	J Proteomics; Congenital cataracts affect the retinal visual cycle and mitochondrial function: A multi-omics study of GJA8 knockout rabbits; Authors - Weijie Sun
human	WB	Adv Sci (Weinh); Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non-Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production; Authors - Qi-Gang Zeng
		Proc Natl Acad Sci U S A; The mitochondrial ATP synthase is a negative regulator of the mitochondrial permeability transition pore; Authors - Ryan Pekson

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

P (Verified Customer) (05-16-2024)

Good

• Applications: Western Blot
• Cell Tissue Type: Ac16

ATP5J Antibody Western Blot validation ( dilution) in Ac16 (Cat no:14114-1-AP)