ATP5J2 Monoklonaler Antikörper

ATP5J2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2B3C3

Kat-Nr. : 68128-1-Ig

Synonyme

ATP5J2, ATP5JL

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  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit heart using 68128-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 68128-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, Mausherzgewebe, Mauslebergewebe, Kaninchenherzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

68128-1-Ig bindet in WB, IHC, IF/ICC, ELISA ATP5J2 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP5J2 fusion protein Ag8811
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
• Berechnetes Molekulargewicht: 5-11 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC003678
• Gene symbol: ATP5J2
• Gene ID (NCBI): 9551
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration; Authors - Wenya Ma
human	WB,IHC	J Transl Med; Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC; Authors - Kaiyan Chen
human	WB	Cell; YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies; Authors - Zhenhua Chen