ATP5O Polyklonaler Antikörper

ATP5O Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 10994-1-AP

Synonyme

ATP5PO, ATP synthase peripheral stalk subunit OSCP, ATPO, OSCP

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  WB analysis using 10994-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10994-1-AP (ATP5O antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10994-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10994-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10994-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 10994-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10994-1-AP (OSCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10994-1-AP (OSCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10994-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10994-1-AP (ATP5O antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 10994-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ATP5O Polyclonal antibody (10994-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, HCT116-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maus-Kolongewebe, Mausherzgewebe, Mauslebergewebe, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

10994-1-AP bindet in WB, IHC, FC (Intra), ELISA ATP5O und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP5O fusion protein Ag1458
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC021233
• Gene symbol: ATP5O
• Gene ID (NCBI): 539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.

Publikationen

Species	Application	Title
human	IHC	Cancers (Basel); Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer.; Authors - Robert Wiebringhaus
human	WB	Neural Regen Res; CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson's disease; Authors - Xiang Chen
human	WB	Brain Pathol; Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.; Authors - Margalida A Frau-Méndez
human	WB	J Mol Biol; COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.; Authors - David Pacheu-Grau
human	WB	Dis Markers; CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.; Authors - Yingyan Han
		Oncol Lett; Lentivirus-mediated silencing of MPHOSPH8 inhibits MTC proliferation and enhances apoptosis.; Authors - Peiyong Li