ATP5O Monoklonaler Antikörper

ATP5O Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D3B8

Kat-Nr. : 66696-1-PBS

Synonyme

ATP5PO, 1D3B8, ATP synthase peripheral stalk subunit OSCP, ATPO, OSCP

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  WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-Ig (ATP5O antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66696-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATP5O und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP5O fusion protein Ag1458
• Vollständiger Name: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC021233
• Gene symbol: ATP5O
• Gene ID (NCBI): 539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.