ATP6V0A1 Polyklonaler Antikörper

ATP6V0A1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 13828-1-AP

Synonyme

a1, ATP6N1, ATP6N1A, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Stv1

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  WB analysis using 13828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13828-1-AP (ATP6V0A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13828-1-AP

  IP result of anti-ATP6V0A1 (IP:13828-1-AP, 4ug; Detection:13828-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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  IHC staining of mouse brain using 13828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13828-1-AP (ATP6V0A1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13828-1-AP (ATP6V0A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13828-1-AP (ATP6V0A1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13828-1-AP bindet in WB, IHC, IF, IP, ELISA ATP6V0A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V0A1 fusion protein Ag4191
• Vollständiger Name: ATPase, H+ transporting, lysosomal V0 subunit a1
• Berechnetes Molekulargewicht: 837 aa, 96 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC032398
• Gene symbol: ATP6V0A1
• Gene ID (NCBI): 535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation(PMID: 7774924 34904569).

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Life Sci; Golgi pH elevation due to loss of V-ATPase subunit V0a2 function correlates with tissue-specific glycosylation changes and globozoospermia; Authors - Johannes Kopp
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
human	WB,IP	Int J Biol Sci; Toosendanin, a novel potent vacuolar-type H+-translocating ATPase inhibitor, sensitizes cancer cells to chemotherapy by blocking protective autophagy.; Authors - Yu Dong
mouse	WB	Sci Rep; TRPM2 promotes autophagic degradation in vascular smooth muscle cells.; Authors - Qiannan Zhao
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Mol Immunol; Systematic deletion of adenosine receptors reveals novel roles in inflammation and pyroptosis in THP-1 macrophages.; Authors - Brandon J Eudy