ATP6V0A4 Polyklonaler Antikörper

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ATP6V0A4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21570-1-PBS

Synonyme

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  WB analysis using 21570-1-AP (same clone as 21570-1-PBS)

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 21570-1-AP (same clone as 21570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21570-1-PBS bindet in WB, IHC, Indirect ELISA ATP6V0A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V0A4 fusion protein Ag16095
• Vollständiger Name: ATPase, H+ transporting, lysosomal V0 subunit a4
• Berechnetes Molekulargewicht: 840 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC109305
• Gene symbol: ATP6V0A4
• Gene ID (NCBI): 50617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.