ATP6V0A4 Rekombinanter Antikörper

ATP6V0A4 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250861E8

Kat-Nr. : 86321-1-RR

Synonyme

A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C

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  WB analysis of rat kidney using 86321-1-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86321-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:80000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:80000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86321-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86321-1-RR against Human ATP6V0A4 were performed. The affinity constant is 0.715 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:10000-1:40000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86321-1-RR bindet in WB, IHC, ELISA ATP6V0A4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP6V0A4 fusion protein Ag16095
• Vollständiger Name: ATPase, H+ transporting, lysosomal V0 subunit a4
• Berechnetes Molekulargewicht: 840 aa, 96 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC109305
• Gene symbol: ATP6V0A4
• Gene ID (NCBI): 50617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness.