ATP6V0D1 Polyklonaler Antikörper

ATP6V0D1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 18274-1-AP

Synonyme

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

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Galerie der Validierungsdaten

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  WB analysis using 18274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18274-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 18274-1-AP

  IP result of anti-ATP6V0D1(IP:18274-1-AP, 4ug; Detection:18274-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human kidney using 18274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18274-1-AP

  Immunofluorescent analysis of HeLa cells using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18274-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (18274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18274-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ATP6V0D1 antibody (18274-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Maushodengewebe, Mausnierengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

18274-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ATP6V0D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V0D1 fusion protein Ag13002
• Vollständiger Name: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• Berechnetes Molekulargewicht: 351 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC008861
• Gene symbol: ATP6V0D1
• Gene ID (NCBI): 9114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Publikationen

Species	Application	Title
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
mouse	WB	Autophagy; Disruption of the vacuolar-type H(+)-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.; Authors - Sandra Kissing
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
		Cells; A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c; Authors - Christian Lévêque
human	WB,IF	J Biol Chem; Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts.; Authors - Otomo Takanobu T