ATP6V0D1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP6V0D1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 18274-1-AP

Synonyme

V ATPase subunit d 1, V ATPase AC39 subunit, p39, ATP6DV, ATP6D

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  WB analysis using 18274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18274-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 18274-1-AP

  IP result of anti-ATP6V0D1 (IP:18274-1-AP, 4ug; Detection:18274-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human kidney using 18274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18274-1-AP

  Immunofluorescent analysis of HeLa cells using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18274-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (18274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 2 publications below

Produktinformation

18274-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ATP6V0D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V0D1 fusion protein Ag13002
• Vollständiger Name: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• Berechnetes Molekulargewicht: 351 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC008861
• Gene symbol: ATP6V0D1
• Gene ID (NCBI): 9114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Metab; Time of exercise differentially impacts bone growth in mice; Authors - Shaoling Yu
human	WB	Autophagy; Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency; Authors - Fangyang Wang
mouse	WB	Autophagy; Disruption of the vacuolar-type H(+)-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.; Authors - Sandra Kissing
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
		Cells; A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c; Authors - Christian Lévêque

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zakia (Verified Customer) (05-22-2024)

we are using this for our lab, Works great,

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: N2A,293T cells, ReNcells

ATP6V0D1 Antibody Western Blot,Immunohistochemistry validation (1:500 dilution) in N2A,293T cells, ReNcells (Cat no:18274-1-AP)