ATP6V0D1 Monoklonaler Antikörper

ATP6V0D1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7A12

Kat-Nr. : 68506-1-PBS

Synonyme

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

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  WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

  IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68506-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ATP6V0D1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP6V0D1 fusion protein Ag13012
• Vollständiger Name: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• Berechnetes Molekulargewicht: 351 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC008861
• Gene symbol: ATP6V0D1
• Gene ID (NCBI): 9114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.