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ATP6V1A Polyklonaler Antikörper

ATP6V1A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(44)

Kat-Nr. : 17115-1-AP

Synonyme

V-ATPase subunit A, V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, Daudi-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, Mausnierengewebe, Maushodengewebe, Rattenhodengewebe, U-87 MG-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Pankreasgewebe, humanes Schilddrüsenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCNIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17115-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ATP6V1A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ATP6V1A fusion protein Ag10801
Vollständiger Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Berechnetes Molekulargewicht 617 aa, 68 kDa
Beobachtetes Molekulargewicht 68 kDa
GenBank-ZugangsnummerBC013138
Gene symbol ATP6V1A
Gene ID (NCBI) 523
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ATP6V1A antibody 17115-1-APProtokoll herunterladen
IHC protocol for ATP6V1A antibody 17115-1-APProtokoll herunterladenl
IF protocol for ATP6V1A antibody 17115-1-APProtokoll herunterladen
IP protocol for ATP6V1A antibody 17115-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Authors - Zharko Daniloski
mouseWB

Nat Immunol

Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.

Authors - Rui Martins
humanWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle
humanWB

Autophagy

Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency

Authors - Fangyang Wang
human,mouseWB

Mol Psychiatry

Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.

Authors - A Pérez-Cañamás
humanWB

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/antibody dilution solution: 0.05% saponin, 5% horse serum in PBS. Primary antibody diluted 1:100 in saponin solution for 1hr at room temperature, followed by anti-rabbit conjugated to AlexaFluor 488 (1:200 in saponin solution) for 1 hr at room temperature.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: MDA-MB-231 cells
ATP6V1A Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:17115-1-AP)