ATP6V1B1 Polyklonaler Antikörper

ATP6V1B1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14780-1-AP

Synonyme

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

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  WB analysis using 14780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14780-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14780-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 14780-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (14780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Mausnierengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

14780-1-AP bindet in WB, IHC, IF/ICC, ELISA ATP6V1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1B1 fusion protein Ag6332
• Vollständiger Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC063411
• Gene symbol: ATP6V1B1
• Gene ID (NCBI): 525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
mouse	IF	Dis Model Mech; Expression of N471D strumpellin leads to defects in the endolysosomal system.; Authors - Lin Song
human	WB	J Cell Physiol; Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.; Authors - Milena Kluge
mouse	WB	Environ Int; Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury; Authors - Xing Zhang
human	WB,IHC	Mol Cell Biochem; ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway; Authors - Shien Mo ; KD Validated
mouse	IF	Physiol Rep; Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract; Authors - Marianela G Dalghi