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ATP6V1B1 Monoklonaler Antikörper

ATP6V1B1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10A8

Kat-Nr. : 68219-1-PBS

Synonyme

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

Filter:
  • Western Blot (WB) analysis of various lysates using ATP6V1B1 Monoclonal antibody (68219-1-Ig)

    WB analysis using 68219-1-Ig (same clone as 68219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68219-1-Ig (ATP6V1B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68219-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP6V1B1 Monoclonal antibody (68219-1-Ig)

    IF Staining of HEK-293 using 68219-1-Ig (same clone as 68219-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (68219-1-Ig, Clone: 1G10A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68219-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68219-1-PBS bindet in WB, IF/ICC, Indirect ELISA ATP6V1B1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ATP6V1B1 fusion protein Ag7405
Vollständiger Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Berechnetes Molekulargewicht 57 kDa
Beobachtetes Molekulargewicht56 kDa
GenBank-ZugangsnummerBC063411
Gene symbol ATP6V1B1
Gene ID (NCBI) 525
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.