ATP6V1B1 Rekombinanter Antikörper

ATP6V1B1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240955B9

Kat-Nr. : 83953-5-RR

Synonyme

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

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  WB analysis using 83953-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 83953-5-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 83953-5-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83953-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 83953-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Rattennierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83953-5-RR bindet in WB, IHC, ELISA ATP6V1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP6V1B1 fusion protein Ag6332
• Vollständiger Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC063411
• Gene symbol: ATP6V1B1
• Gene ID (NCBI): 525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.