ATP6V1B2 Monoklonaler Antikörper

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ATP6V1B2 Monoklonal Antikörper für Indirect ELISA, IP, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

Indirect ELISA, IP, WB

Konjugation

Unkonjugiert

CloneNo.

3C11A9

Kat-Nr. : 68441-1-PBS

Synonyme

V-ATPase subunit B 2, VATB, HO57, ATP6B2, ATP6B1B2

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  WB analysis using 68441-1-Ig (same clone as 68441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68441-1-Ig (ATP6V1B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68441-1-Ig (same clone as 68441-1-PBS)

  WB result of ATP6V1B2 antibody (68441-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1B2 transfected A549 cells. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

  IP result of anti-ATP6V1B2 (IP:68441-1-Ig, 4ug; Detection:68441-1-Ig 1:4000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68441-1-Ig (same clone as 68441-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ATP6V1B2 Monoclonal antibody (68441-1-Ig, Clone:3C11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68441-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68441-1-PBS bindet in Indirect ELISA, IP, WB ATP6V1B2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ATP6V1B2 fusion protein Ag7265
• Vollständiger Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 56-58 kDa
• GenBank-Zugangsnummer: BC003100
• Gene symbol: ATP6V1B2
• Gene ID (NCBI): 526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).