ATP6V1C1 Polyklonaler Antikörper

ATP6V1C1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 16054-1-AP

Synonyme

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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  WB analysis of NIH/3T3 using 16054-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 16054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16054-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16054-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 16054-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 16054-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

16054-1-AP bindet in WB, IHC, ELISA ATP6V1C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1C1 fusion protein Ag9020
• Vollständiger Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Berechnetes Molekulargewicht: 382 aa, 44 kDa
• Beobachtetes Molekulargewicht: 42-44 kDa
• GenBank-Zugangsnummer: BC010960
• Gene symbol: ATP6V1C1
• Gene ID (NCBI): 528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).

Publikationen

Species	Application	Title
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong
mouse	WB	J Biol Chem; A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.; Authors - Kie Itoh
human	WB	Commun Biol; A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.; Authors - Masashi Watanabe
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
human	IHC	Bioengineered; A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.; Authors - Huimin Shen
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou