ATP6V1C1 Rekombinanter Antikörper

ATP6V1C1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250958C3

Kat-Nr. : 86345-2-PBS

Synonyme

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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  WB analysis using 86345-2-RR (same clone as 86345-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  Sample test of MP02350-1

  The mean ATP6V1C1 concentration was determined to be 29.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02350-1

  Cytometric bead array standard curve of MP02350-1, ATP6V1C1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86345-2-PBS. Detection antibody: 86345-1-PBS. Standard: Ag9020. Range: 1.562-200 ng/mL.

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  Affinity and Kinetic Characterization of 86345-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86345-2-RR against Human ATP6V1C1 were performed. The affinity constant is 8.06 nM.

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Geprüfte Anwendungen

Produktinformation

86345-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA, Sample test ATP6V1C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP6V1C1 fusion protein Ag9020
• Vollständiger Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Berechnetes Molekulargewicht: 382 aa, 44 kDa
• Beobachtetes Molekulargewicht: 42-44 kDa
• GenBank-Zugangsnummer: BC010960
• Gene symbol: ATP6V1C1
• Gene ID (NCBI): 528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).