ATP6V1C1 Rekombinanter Antikörper

ATP6V1C1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250958C3

Kat-Nr. : 86345-2-RR

Synonyme

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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  WB analysis using 86345-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 86345-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 86345-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86345-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86345-2-RR against Human ATP6V1C1 were performed. The affinity constant is 8.06 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe, NIH/3T3-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86345-2-RR bindet in WB, IHC, ELISA ATP6V1C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATP6V1C1 fusion protein Ag9020
• Vollständiger Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Berechnetes Molekulargewicht: 382 aa, 44 kDa
• Beobachtetes Molekulargewicht: 42-44 kDa
• GenBank-Zugangsnummer: BC010960
• Gene symbol: ATP6V1C1
• Gene ID (NCBI): 528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).