ATP6V1D Polyklonaler Antikörper

ATP6V1D Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14920-1-AP

Synonyme

ATP6M, ATP6V1D, V ATPase subunit D, V type proton ATPase subunit D, Vacuolar proton pump subunit D, VATD, VMA8

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Galerie der Validierungsdaten

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  WB analysis of human brain using 14920-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14920-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14920-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 14920-1-AP

  IP Result of anti-ATP6V1D (IP:14920-1-AP, 4ug; Detection:14920-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 14920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 10x lens).

• 

  FC experiment of HeLa using 14920-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ATP6V1D antibody (14920-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Mauslungengewebe, Maus-Skelettmuskelgewebe, Rattenlungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

14920-1-AP bindet in WB, IP, IHC, FC, ELISA ATP6V1D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1D fusion protein Ag6737
• Vollständiger Name: ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC001411
• Gene symbol: ATP6V1D
• Gene ID (NCBI): 51382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1D is also named as ATP6M, VATD(V-type proton ATPase subunit D) and belongs to the V-ATPase D subunit family. ATP6V1D gene has been under strong negative selection during evolution and is highly conserved among mammals, flies, worms, yeast, plants, and bacteria(PMID:11435709). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
rat	WB	J Proteome Res; Quantitative proteome of medulla oblongata in spontaneously hypertensive rats.; Authors - Sun Dongmei D
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou
human	WB	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh