ATP6V1F Polyklonaler Antikörper

ATP6V1F Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17725-1-PBS

Synonyme

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  WB analysis of MCF-7 using 17725-1-AP (same clone as 17725-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17725-1-AP (same clone as 17725-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 17725-1-AP (same clone as 17725-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 17725-1-AP (same clone as 17725-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 17725-1-AP (same clone as 17725-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17725-1-AP (ATP6V1F Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17725-1-AP (ATP6V1F Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 17725-1-AP (same clone as 17725-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17725-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17725-1-PBS bindet in WB, IHC, Indirect ELISA ATP6V1F und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1F fusion protein Ag12121
• Vollständiger Name: ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
• Berechnetes Molekulargewicht: 119 aa, 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC107854
• Gene symbol: ATP6V1F
• Gene ID (NCBI): 9296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.