ATP6V1H Polyklonaler Antikörper

ATP6V1H Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 26683-1-AP

Synonyme

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 26683-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26683-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 26683-1-AP

  IP Result of anti-ATP6V1H (IP:26683-1-AP, 4ug; Detection:26683-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below

Produktinformation

26683-1-AP bindet in WB, IP, ELISA ATP6V1H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP6V1H fusion protein Ag24688
• Vollständiger Name: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Berechnetes Molekulargewicht: 483 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC025275
• Gene symbol: ATP6V1H
• Gene ID (NCBI): 51606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	Int J Mol Sci; Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway; Authors - Zanyan Zhao
human	WB	Front Immunol; Acidic Microenvironment Regulates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating the Generation and Function of Tregs via the PI3K-mTOR Pathway.; Authors - Xiaojie Gan
rat	WB	J Cell Physiol; A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.; Authors - Vishwanatha K Rao
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li