ATP7B-Specific Rekombinanter Antikörper

ATP7B-Specific Rekombinant Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242827B3

Kat-Nr. : 85288-2-PBS

Synonyme

ATP7B, 242827B3, ATPase, Cu++ transporting, beta polypeptide, Copper-transporting ATPase 2, EC:7.2.2.8

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  WB analysis using 85288-2-RR (same clone as 85288-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ATP7B-Specific antibody (85288-2-RR, Clone: 242827B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 85288-2-RR (same clone as 85288-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ATP7B-Specific Recombinant antibody (85288-2-RR, Clone:242827B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85288-2-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA ATP7B-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATPase, Cu++ transporting, beta polypeptide
• Berechnetes Molekulargewicht: 157 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_000053
• Gene symbol: ATP7B
• Gene ID (NCBI): 540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.