ATP8A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP8A1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (1)

Kat-Nr. : 21565-1-AP

Synonyme

ATP8A1, ATPase II, ATPASEII, ATPIA, ATPP2, Chromaffin granule ATPase II

---

• 

  WB analysis of mouse brain using 21565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21565-1-AP (ATP8A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21565-1-AP (ATP8A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IF: See 3 publications below

Produktinformation

21565-1-AP bindet in WB, IF, ELISA ATP8A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP8A1 fusion protein Ag16071
• Vollständiger Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
• Berechnetes Molekulargewicht: 1164 aa, 131 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC109317
• Gene symbol: ATP8A1
• Gene ID (NCBI): 10396
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO J; Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.; Authors - Shoken Lee
human	WB, IF	Proc Natl Acad Sci U S A; AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells.; Authors - Seunghyi Kook
mouse	WB	Sci Rep; Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues.; Authors - Jiao Wang
human	WB	Sci Rep; PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation; Authors - Chiaki Inoue ; KD Validated
human	WB	iScience; Lipid flippase dysfunction as a therapeutic target for endosomal anomalies in Alzheimer's disease.; Authors - Nanaka Kaneshiro
human	WB	Clin Proteomics; Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization; Authors - Ondrej Vit

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Mareike (Verified Customer) (01-17-2025)

The sample must not be boiled before application!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human breast cancer cell line MDA-MB-231 as wild type and Atp8a1 overexpression cell line

ATP8A1 Antibody Western Blot validation (1:1000 dilution) in human breast cancer cell line MDA-MB-231 as wild type and Atp8a1 overexpression cell line (Cat no:21565-1-AP)