ATP8A2 Polyklonaler Antikörper

ATP8A2 Polyklonal Antikörper für IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31697-1-AP

Synonyme

ML 1, ATPIB

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  IP experiment of mouse testis using 31697-1-AP

  IP result of anti-ATP8A2 (IP:31697-1-AP, 4ug; Detection:31697-1-AP 1:600) with mouse testis tissue lysate 1120 ug.

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  IHC staining of mouse brain using 31697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 31697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31697-1-AP bindet in IHC, IP, ELISA ATP8A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP8A2 fusion protein Ag36276
• Vollständiger Name: ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
• Berechnetes Molekulargewicht: 133kDa
• Beobachtetes Molekulargewicht: 129 kDa
• GenBank-Zugangsnummer: NM_001313741
• Gene symbol: ATP8A2
• Gene ID (NCBI): 51761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP8A2 is also known as ATPIB. ATP8A2 is a member of the P4‐ATPase subfamily of P‐type ATPases that actively flips phosphatidylserine (PS) and phosphatidylethanolamine (PE) across membranes to generate and maintain transmembrane phospholipid asymmetry, a property important in such cellular processes as vesicle trafficking, apoptosis, cytokinesis, neuron survival, blood coagulation, and phagocytosis. ATP8A2 consists of an N‐domain involved in binding ATP, a P‐domain that undergoes phosphorylation, an A‐domain that functions in the dephosphorylation of the phosphorylated intermediate, and an M‐domain consisting of 10 transmembrane segments that comprise the pathway for translocation of the lipid across the membrane. ATP8A2 is highly expressed in the brain, spinal cord, testis, and retina (PMID: 33565221, PMID: 33766557, PMID: 37635783).