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ATPB Monoklonaler Antikörper

ATPB Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11B9

Kat-Nr. : 66600-1-PBS

Synonyme

1A11B9, ATP synthase F1 subunit beta, ATP5B, ATP5F1B, ATPMB

Filter:
  • Western Blot (WB) analysis of various lysates using ATPB Monoclonal antibody (66600-1-Ig)

    WB analysis using 66600-1-Ig (same clone as 66600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66600-1-Ig (ATPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of mouse skeletal muscle using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of mouse skeletal muscle using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ATPB Monoclonal antibody (66600-1-Ig)

    IF Staining of U2OS using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATPB Monoclonal antibody (66600-1-Ig)

    IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATPB Monoclonal antibody (66600-1-Ig)

    IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66600-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ATPB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ATPB fusion protein Ag11177
Vollständiger Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Berechnetes Molekulargewicht 529 aa, 57 kDa
Beobachtetes Molekulargewicht45-57 kDa
GenBank-ZugangsnummerBC016512
Gene symbol ATPB
Gene ID (NCBI) 506
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.