ATPBD3 Rekombinanter Antikörper

ATPBD3 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241630C4

Kat-Nr. : 84318-4-PBS

Synonyme

NCS6, CTU1, 241630C4

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  WB analysis using 84318-4-RR (same clone as 84318-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84318-4-RR (ATPBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ATPBD3 antibody (84318-4-RR, Clone: 241630C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84318-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84318-4-RR against Human ATPBD3 were performed. The affinity constant is 0.406 nM.

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Geprüfte Anwendungen

Produktinformation

84318-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA ATPBD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ATPBD3 fusion protein Ag35037
• Vollständiger Name: ATP binding domain 3
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_145232
• Gene symbol: ATPBD3
• Gene ID (NCBI): 90353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.